Table 3.
Effect of cytokinins (BA and Kin) on shoot multiplication through repeated transfer of mother explants of E. foliata
| BA (mg l−1) | Kin (mg l−1) | Number of shoots (mean ± SD) | Shoot length (cm) (mean ± SD) |
|---|---|---|---|
| 0.00 | 0.00 | 1.5 ± 0.53j | 0.70 ± 0.48i |
| 0.25 | 0.00 | 4.5 ± 0.9 hi | 1.92 ± 0.90gh |
| 0.50 | 0.00 | 5.1 ± 0.74h | 2.24 ± 0.84fg |
| 0.75 | 0.00 | 9.4 ± 0.97f | 2.81 ± 0.42ef |
| 1.00 | 0.00 | 14.2 ± .79e | 3.13 ± 0.62de |
| 0.00 | 0.25 | 5.2 ± 0.42h | 1.45 ± 0.59h |
| 0.00 | 0.50 | 7.3 ± 0.78g | 1.86 ± 0.77h |
| 0.00 | 0.75 | 6.9 ± 0.87g | 2.37 ± 0.99fg |
| 0.00 | 1.00 | 8.7 ± 0.67f | 2.86 ± 0.90ef |
| 0.25 | 0.25 | 15.1 ± 0.99d | 3.64 ± 0.93cd |
| 0.50 | 0.25 | 18.2 ± 0.92b | 4.57 ± 0.63b |
| 0.75 | 0.25 | 19.9 ± 0.99a | 5.48 ± 0.66a |
| 1.00 | 0.25 | 17.3 ± 0.94c | 4.13 ± 0.31bc |
Medium: MMS2 + additives. Passage of repeated transfer of mother explants: 3rd. Data were recorded after 4 weeks of culture. Means in each column followed by same letters are not significantly different according to DMRT at P < 0.05