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. Author manuscript; available in PMC: 2014 Jul 17.
Published in final edited form as: Insect Mol Biol. 2012 Dec 13;22(1):18–30. doi: 10.1111/imb.12005

Figure 3. Stage-specific expression of the β-galactosidase reporter in the 2M2 transgenic line.

Figure 3

Frozen mosquitoes of the indicated developmental stages from the 2M2 line and the WE control were assayed for β-galactosidase (β-gal) activity (as in Fig. 2). Third-instar (L3) larvae (3 samples of ~30 larvae each) and a mixture of L1 and L2 larvae were collected. Individual L4 larvae (~48 h after L3/L4 molt; n=3) were dissected and the abdomens assayed for β-gal activity; RNA isolated from the head and thorax of each larva was used to assay its sex by RT-PCR as described in Experimental procedures. Individual pupae (n=3) were assayed in repeated experiments at 5 and 46 h after the larval/pupal molt. Three (3) groups of 5 adult animals pooled were assayed at days 5 and 10 after eclosion.