. Author manuscript; available in PMC: 2014 Jul 17.

Published in final edited form as: Insect Mol Biol. 2012 Dec 13;22(1):18–30. doi: 10.1111/imb.12005

Table 2.

Comparison of relative lacZ mRNA expression at different developmental stages of the 2M2 transgenic line.

Developmental stage	Fold difference between females and males	p
L4 larvae	1.11^b	0.85
P5 pupae	1.3^a	0.48
Ad5 adults	3.58^b	0.03^*
Bloodfed females^*	-2.49^a	0.22
	-1.91^b	0.45

Levels of the Adh-lacZ-SV40 transcript in female vs. male 2M2 mosquitoes, of the indicated developmental stages, were analyzed by real-time PCR, relative to α-tubulin or β-actin mRNA levels. Primers (lacZ-SV40 bridge) and qPCR conditions are described in Experimental procedures and Table S1.

In a 1-way ANOVA performed for each stage, a significant p value was only observed for 5-day old adults. Bloodfed females were compared to unfed females.

Δ ^tub data used in statistical analysis;

Δ ^act data.