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. 2014 Jun 28;15(1):535. doi: 10.1186/1471-2164-15-535

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© Zuleta et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phylogenetic trees generated for the NifHDK (A) and NifEN (B) concatenated amino acid sequences from the analysed Burkholderia species and some Rhizobiales species. The sequences were aligned and submitted to the MEGA5 program, using the maximum likelihood method and WAG + G + I (A) and JTT + G (B) models, with 5 rate categories. All positions showing less than 95% site coverage were eliminated. Bootstrap values were based on 1000 replicates. Cupriavidus taiwanensis was used as outgroup.