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. 2014 Jul 4;47(1):31. doi: 10.1186/0717-6287-47-31

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© Urriola-Muñoz et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Colocalization of ADAM10 and ADAM17 in isolated germ and Sertoli cells. Isolated germ and Sertoli cells were double labelled with anti-ADAM10 (green) and anti-ADAM17 (red). ADAM10 and ADAM17 colocalise in pachytene, round spermatids and Sertoli cells.). Mature caudal epididymis sperm showed immunoreactions against ADAM10 and ADAM17 antibodies in the head (arrowhead) and tail. The last column shows the negative controls (without the primary antibody) merged with the bright field picture. Bars in spermatocyte and round spermatids: 5 μm, sperm: 20 μm, and Sertoli cell: 50 μm.