Fig. 1.

Ganetespib activity in HCT 116 colon cancer cells in vitro and in vivo. a HCT 116 cells were treated with increasing concentrations of ganetespib or 5-FU and cell viability assessed after 72 h. b HCT 116 cells were exposed to graded concentrations of ganetespib or vehicle (V) for 24 h as indicated. Cell lysates were immunoblotted using antibodies against MET and phosphorylated Src (p-Src) as shown. GAPDH was included as a loading control. c HCT 116 cells were exposed to vehicle or ganetespib (25, 50 and 100 nM) for 24 h as indicated. Cell lysates were immunoblotted using antibodies against EGFR, IGF-1R, phosphorylated ERK (p-ERK), total ERK, phosphorylated AKT (p-AKT), total AKT, and phosphorylated 4E-BP1 (p-4E-BP1). d Mice bearing established HCT-116 xenografts (n = 8/group) were i.v. dosed with 150 mg/kg ganetespib once weekly over a 3 week cycle. % T/C values are indicated to the right of each growth curve and the error bars are the SEM; (*, p < 0.05)