Fig. 2.

Ganetespib modulates cell cycle expression and induces growth arrest and apoptosis in HCT 116 colon cancer cells. a HCT 116 cells were treated with increasing concentrations of ganetespib as indicated. Cell cycle distribution was determined by flow cytometry 18 h post-treatment. b HCT 116 cells were treated with graded concentrations of ganetespib for 18 h. Cell lysates were immunoblotted using antibodies against CHK1, CHK2, p21 and p27. Arrow depicts higher molecular weight form of CHK2 induced following ganetespib treatment. c HCT 116 cells were exposed to increasing concentrations of ganetespib for 24 h. Cell lysates were immunoblotted using antibodies against RAD51, phosphorylated histone H2AX (p-histone H2AX), Bcl-XL and RIP, as indicated. d Quantification of apoptosis as assessed by Annexin V positivity. HCT 116 cells were exposed to increasing concentrations of ganetespib for 24 h and Annexin V/PI staining measured by flow cytometry