Fig. 3.

Deletion mutants show altered static biofilm formation compared to the wild type (WT) parental strain. Biofilms of WT, deletion mutants, and mutants and WT with control or complementation plasmids, as indicated, were quantified after 24 h, 48 h and 72 h. In detail, impact on static biofilm formation of VC0374 (A), VC0433 (B), VC2750 (C), VCA0686 (D), VCA0785 (E) and VCA0955 (E) is shown. The biofilm formation capacity was assayed under static conditions by crystal violet staining and subsequent determination of the OD₅₉₅. Shown are the medians from at least eight independent measurements. The error bars indicate the interquartile range. An asterisk on top of the respective mutant data set indicates a significant difference to the WT (*P < 0.05 by Mann–Whitney U test). An asterisk on top of the mutant with control plasmid indicates a significant difference to the WT with control plasmid and an asterisk on top of the mutant with complementation plasmid indicates a significant difference to the mutant with control plasmid, respectively (*P < 0.05 by Kruskal–Wallis test followed by post hoc Dunn's multiple comparisons).