Fig. 4.

Images of dynamically grown flow cell biofilms of mutants with altered biofilm amount or morphology compared to wild type (WT). Shown are confocal laser scanning microscopy images of GFP expressing WT and mutant biofilms as horizontal (xy) and vertical (xz and yz) projections (large and side panels, respectively). Biofilms were grown for 24 h in flow cell chambers with constant 2% LB medium flow. Large panels represent selected single optical sections through the acquired three-dimensional data sets at the indicated z position. Optical sectioning was performed in 0.13 μm steps.