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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biomaterials. 2014 Jun 21;35(28):8134–8143. doi: 10.1016/j.biomaterials.2014.06.009

Fig. 6.

Fig. 6

Control of human VEGF165 transgene expression in plasmonic hydrogels. (a) C3H/T101/2-VEGF cells were encapsulated within hydrogels containing (+) or not (−) 0.03 mg mL−1 HGNPs and cultured for 24 h. Constructs were treated with rapamycin, exposed to NIR irradiation at 44 mW mm−2 for the indicated times and cultured further for 24 h. Media were collected and VEGF secretion was measured. (b) Hydrogels containing the indicated doses of HGNPs and harboring C3H/10T1/2-VEGF cells were NIR-irradiated at 17 mW mm−2 at two separate locations for 12.5 min each. Left panel: implantation areas were photographed 4 days after NIR irradiation. Dashed circles in magnified images indicate laser incidence areas. Insets show thermograms of implantation areas after NIR irradiation. Arrows indicate the site of hydrogel injection. Right panel: the graph shows the extent of erythematous skin lesions, relative to the total surface area of the implant. Scale bars = 1 cm. N.D.: not detected.