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. 2014 Apr 17;4(2):402–418. doi: 10.3390/biom4020402

Figure 7.

Figure 7

Synthetic two-component pathway that combines redesigned receptor (blue) with chimeric histidine kinase (HK) consisting of periplasmic domain from chemotaxis sensor (pink) and cytoplasmic domain from osmosensor (grey). (A) This chimeric HK phosphorylates response regulator (RR, grey) from osmosensor, which then turns on transcription of the β-galactosidase gene (green); (B) Reaction used to measure β-galactosidase activity. ONPG, 2-nitrophenyl-β-galactopyranoside.