Table 4.

Intra and extracellular amino acid concentrations in P. pastoris cells growing on glucose/methanol. Intracellular concentrations (Intra.) of metabolites pools are given in μmol/g_CDW. Extracellular concentrations (Extra.) of metabolites are given in μmol/L.

Amino acid	X-33 Control				X-33/ROL
	Intra. (μmol/gDCW)		Extra. (μmol/L)		Intra. (μmol/gDCW)		Extra. (μmol/L)
	Value	Sd	Value	Sd	Value	Sd	Value	Sd
Glu	84.85	2.97	n.d.	n.d.	91.98	4.21	0.03	0.04
Gln	84.97	2.40	0.01	0.00	88.40	3.54	26.05	36.68
Asp	39.56	0.56	0.08	0.00	45.58	3.58	0.03	0.04
Orn	22.59	1.87	1.53	1.10	28.28	1.75	0.16	0.32
Ala	15.01	1.21	1.19	0.22	13.56	0.71	2.77	3.50
Lys	10.22	0.18	0.27	0.22	12.27	0.44	1.15	1.77
Ser	5.94	1.20	n.d	n.d.	9.30	0.38	0.53	0.38
Asn	4.66	0.10	0.11	0.08	9.64	0.39	0.39	0.48
His	4.79	0.15	0.01	0.02	8.13	0.32	0.30	0.43
Gly	1.33	2.44	0.44	0.33	2.35	0.07	2.02	0.87
Thr	2.49	0.21	0.04	0.02	4.24	0.16	0.25	0.04
Pro	2.61	0.07	0.03	0.03	3.85	0.15	0.25	0.16
Val	1.30	0.14	n.d.	n.d.	2.41	0.17	0.24	0.36
Leu	0.69	0.23	0.06	0.04	1.55	0.22	0.39	0.52
Ile	0.33	0.14	0.04	0.02	0.53	0.11	0.22	0.25
Tyr	0.20	0.10	0.03	0.02	1.02	0.04	0.14	0.20
Phe	0.20	0.13	0.02	0.01	0.65	0.09	0.14	0.20
Met	0.48	0.05	n.d.	n.d.	0.53	0.04	0.04	0.07
Trp	0.09	0.03	n.d.	n.d.	0.33	0.02	0.08	0.14