Table 1.
Metabolite | PCC6803 | PCC7002 | PCC7942 | |||
---|---|---|---|---|---|---|
Average | S.D. | Average | S.D. | Average | S.D. | |
2-Isopropylmalate | 4.11 × 10 −1 | 7.75 × 10 −2 | 1.34 × 10 −1 | 2.01 × 10 −2 | 3.22 × 10 –2 | 1.18 × 10 −2 |
2-Oxoglutarate | 2.94 × 10 −1 | 7.06 × 10 −2 | 1.77 × 100 | 7.23 × 10 −1 | 1.77 × 10 −1 | 5.14 × 10 −2 |
3PGA | 1.48 × 101 | 1.86 × 100 | 2.02 × 101 | 4.87 × 100 | 7.13 × 100 | 1.18 × 100 |
4-Aminobutanoate | 9.67 × 10 −3 | 2.09 × 10 −3 | 9.23 × 10 −3 | 2.40 × 10 −3 | 1.42 × 10 −2 | 1.01 × 10 −2 |
6-Phosphogluconate | 4.66 × 100 | 8.35 × 10 −1 | 1.99 × 100 | 1.11 × 100 | 1.87 × 10 −1 | 4.76 × 10 −2 |
Acetyl CoA | 4.43 × 10 −1 | 4.29 × 10 −2 | 6.22 × 10 −2 | 8.02 × 10 −3 | 6.54 × 10 −2 | 9.12 × 10 −3 |
ADP | 6.51 × 100 | 7.58 × 10 −1 | 2.68 × 100 | 1.46 × 10 −2 | 2.38 × 100 | 6.44 × 10 −1 |
ADP-Glc | 7.00 × 10 −2 | 4.13 × 10 −2 | 6.13 × 10 −1 | 2.66 × 10 −1 | 3.88 × 10 −2 | 1.76 × 10 −2 |
Alanine | 4.53 × 100 | 1.16 × 100 | 6.50 × 10 −1 | 1.48 × 10 −1 | 8.12 × 10 −1 | 6.74 × 10 −1 |
α-Glycerophosphate | 4.77 × 10 −1 | 7.50 × 10 −2 | 9.83 × 10 −1 | 1.94 × 10 −1 | 2.22 × 10 −1 | 3.74 × 10 −2 |
AMP | 1.07 × 101 | 1.48 × 100 | 4.56 × 100 | 4.09 × 10 −1 | 3.92 × 100 | 1.09 × 100 |
Arginine + Ornithine | 2.12 × 10 1 | 5.02 × 100 | 4.31 × 100 | 6.72 × 10 −1 | 3.52 × 100 | 9.79 × 10 −1 |
Asparagine | 4.34 × 10 −1 | 8.01 × 10 −2 | 2.66 × 10 −2 | 3.97 × 10 −3 | 8.68 × 10 −2 | 3.49 × 10 −2 |
Aspartate | 7.14 × 100 | 1.20 × 100 | 2.69 × 100 | 6.09 × 10 -1 | 2.53 × 100 | 1.07 × 100 |
ATP | 2.14 × 100 | 5.58 × 10 −1 | 3.09 × 100 | 3.48 × 10 −1 | 1.90 × 100 | 8.72 × 10 −1 |
Citrate | 2.16 × 100 | 7.83 × 10 −2 | 4.22 × 100 | 1.43 × 100 | 3.57 × 10 −1 | 8.69 × 10 −2 |
CMP | 3.43 × 10 −1 | 7.71 × 10 −2 | 2.17 × 10 −1 | 4.07 × 10 −2 | 2.71 × 10 −1 | 3.67 × 10 −2 |
CTP | 5.97 × 10 −1 | 1.08 × 10 -1 | 6.52 × 10 −2 | 9.81 × 10 −3 | 2.97 × 10 −2 | 1.13 × 10 −2 |
Cystein | 4.79 × 10 −1 | 2.30 × 10 −1 | 5.28 × 10 −1 | 1.73 × 10 −1 | 2.32 × 10 −1 | 1.89 × 10 −1 |
Cytidine | 1.28 × 10 −2 | 4.16 × 10 −3 | 2.11 × 10 −2 | 5.56 × 10 −3 | 3.40 × 10 −2 | 7.35 × 10 −3 |
DHAP | 4.30 × 10 −1 | 7.71 × 10 −2 | 5.48 × 10 −1 | 2.91 × 10 −1 | 1.15 × 10 −1 | 1.67 × 10 −2 |
Disaccharide-P | 6.03 × 10 −4 | 3.27 × 10 −4 | 1.21 × 10 −2 | 9.63 × 10 −3 | 1.56 × 10 −3 | 1.05 × 10 −3 |
F1P | 1.12 × 10 −2 | 3.71 × 10 −3 | 1.90 × 10 −2 | 4.37 × 10 −3 | 6.99 × 10 −3 | 1.04 × 10 −3 |
F6P | 4.61 × 10 −1 | 9.81 × 10 −2 | 5.19 × 10 −1 | 2.13 × 10 −2 | 2.75 × 10 −1 | 7.97 × 10 −2 |
FAD | 9.98 × 10 −2 | 3.19 × 10 −2 | 1.60 × 10 −1 | 3.38 × 10 −2 | 1.98 × 10 −1 | 2.80 × 10 −2 |
FBP | 2.40 × 10 −1 | 1.07 × 10 −1 | 3.39 × 10 −1 | 1.44 × 10 −2 | 2.08 × 10 −2 | 1.37 × 10 −3 |
FMN | 4.77 × 10 −2 | 1.95 × 10 −2 | 4.81 × 10 −2 | 7.94 × 10 −3 | 5.26 × 10 −2 | 2.18 × 10 −2 |
Fumarate | 1.63 × 10 −1 | 2.27 × 10 −2 | 9.08 × 10 −2 | 9.45 × 10 −3 | 5.11 × 10 −2 | 9.58 × 10 −3 |
G1P | 6.91 × 10 −2 | 1.04 × 10 −2 | 1.41 × 10 −1 | 1.45 × 10 −2 | 9.23 × 10 −2 | 4.99 × 10 −2 |
G6P | 1.84 × 100 | 3.10 × 10 −1 | 4.19 × 100 | 7.29 × 10 −1 | 1.57 × 100 | 3.43 × 10 −1 |
GAP | 2.63 × 10 −1 | 7.81 × 10 −2 | 5.59 × 10 −1 | 2.24 × 10 −1 | 1.88 × 10 −1 | 4.91 × 10 −2 |
GDP | 1.38 × 100 | 1.73 × 10 −1 | 9.38 × 10 −1 | 1.77 × 10 −1 | 3.46 × 100 | 4.42 × 100 |
Glutamate | 2.13 × 102 | 4.79 × 101 | 4.25 × 101 | 6.52 × 100 | 3.39 × 101 | 8.11 × 100 |
Glutamine | 3.47 × 100 | 4.61 × 10 −1 | 5.14 × 10 −1 | 1.37 × 10 −2 | 6.34 × 10 −1 | 2.12 × 10 −1 |
Glycerate | 2.53 × 10 −2 | 9.28 × 10 −3 | 2.06 × 10 −2 | 5.33 × 10 −4 | 2.09 × 10 −2 | 1.93 × 10 −2 |
Glycine | 1.67 × 10 1 | 4.51 × 100 | 2.17 × 100 | 8.78 × 10 −1 | 1.78 × 101 | 2.66 × 101 |
Glycolate | 7.51 × 10 −2 | 2.03 × 10 −2 | 3.18 × 10 −2 | 1.47 × 10 −2 | 5.44 × 10 −2 | 1.49 × 10 −2 |
GMP | 7.30 × 10 −1 | 2.05 × 10 −1 | 3.75 × 10 −1 | 3.47 × 10 −2 | 3.77 × 10 −1 | 6.40 × 10 −2 |
GTP | 8.31 × 10 −1 | 2.35 × 10 −1 | 9.61 × 10 −1 | 1.93 × 10 −1 | 4.11 × 10 −1 | 1.31 × 10 −1 |
Guanosine | 7.26 × 10 −2 | 3.51 × 10 −2 | 2.91 × 10 −1 | 1.92 × 10 −1 | 9.43 × 10 −2 | 3.71 × 10 −2 |
Histidine | 1.10 × 10 −1 | 3.46 × 10 −2 | 7.03 × 10 −2 | 2.20 × 10 −2 | 6.45 × 10 −2 | 2.58 × 10 −2 |
HMBPP | 9.27 × 10 −2 | 2.66 × 10 −2 | 5.40 × 10 −2 | 1.90 × 10 −2 | 3.32 × 10 −2 | 4.33 × 10 −3 |
Homoserine | 3.05 × 10 −2 | 1.16 × 10 −2 | 1.32 × 10 −2 | 1.33 × 10 −3 | 1.51 × 10 −2 | 3.85 × 10 −3 |
Hydroxyproline | 9.27 × 10 −1 | 3.05 × 10 −1 | 4.44 × 10 −1 | 1.72 × 10 −1 | 7.65 × 10 −1 | 5.11 × 10 −1 |
IMP | 4.64 × 10 −1 | 3.84 × 10 −2 | 2.48 × 10 −1 | 9.97 × 10 −2 | 1.72 × 10 −1 | 6.00 × 10 −2 |
Inosine | 1.46 × 10 −2 | 5.54 × 10 −3 | 9.41 × 10 −2 | 8.20 × 10 −2 | 6.80 × 10 −2 | 2.51 × 10 −2 |
IPP + DMAPP | 1.40 × 10 −2 | 1.60 × 10 −3 | 9.64 × 10 − 3 | 1.79 × 10 −3 | 1.63 × 10 −2 | 1.28 × 10 −3 |
Isocitrate | 1.45 × 100 | 1.25 × 10 −1 | 3.04 × 100 | 1.43 × 100 | 1.09 × 10 −1 | 4.96 × 10 −3 |
Isoleucine | 3.70 × 10 −1 | 9.37 × 10 −2 | 7.14 × 10 −2 | 1.37 × 10 −2 | 1.50 × 10 −1 | 1.16 × 10 −1 |
Leucine | 7.72 × 10 −1 | 1.55 × 10 −1 | 1.26 × 10 −1 | 2.32 × 10 −2 | 1.76 × 10 −1 | 1.33 × 10 −1 |
Lysine | 1.57 × 10 −1 | 4.40 × 10 −2 | 1.02 × 10 −1 | 1.11 × 10 −2 | 9.32 × 10 −2 | 3.62 × 10 −2 |
Malate | 1.82 × 10 −1 | 2.24 × 10 −2 | 6.36 × 10 −2 | 4.72 × 10 −3 | 2.08 × 10 −1 | 7.92 × 10 −2 |
MEP | 8.38 × 10 −2 | 1.64 × 10 −2 | 3.94 × 10 −2 | 9.17 × 10 −3 | 3.83 × 10 −2 | 8.08 × 10 −3 |
Methionine | 6.13 × 10 −1 | 1.10 × 10 −1 | 2.67 × 10 −1 | 4.40 × 10 −2 | 2.30 × 10 −1 | 4.51 × 10 −2 |
Mn6P | 2.92 × 10 −1 | 4.19 × 10 −2 | 2.53 × 10 −1 | 7.48 × 10 −2 | 2.02 × 10 −1 | 3.06 × 10 −2 |
NAD | 5.14 × 10 −1 | 1.26 × 10 −1 | 3.84 × 10 −1 | 2.97 × 10 −2 | 5.64 × 10 −1 | 1.04 × 10 −1 |
NADP | 6.14 × 10 −1 | 1.26 × 10 −1 | 6.82 × 10 −1 | 1.38 × 10 −1 | 3.79 × 10 −1 | 8.28 × 10 −2 |
Nicotinate | 3.56 × 10 −4 | 7.66 × 10 −5 | 7.72 × 10 −4 | 3.27 × 10 −4 | 4.62 × 10 −4 | 1.86 × 10 −4 |
Orotate | 1.03 × 10 −2 | 1.58 × 10 −3 | 5.97 × 10 −3 | 2.34 × 10 −3 | 1.75 × 10 −3 | 7.23 × 10 −4 |
Pantothenate | 4.91 × 10 −3 | 1.02 × 10 −3 | 2.13 × 10 −4 | 3.22 × 10 −5 | 4.56 × 10 −4 | 2.59 × 10 −4 |
PEP | 2.51 × 100 | 7.59 × 10 −1 | 2.27 × 10 0 | 7.18 × 10 −1 | 1.47 × 100 | 2.45 × 10 −1 |
Phenylalanine | 1.99 × 10 −1 | 6.09 × 10 −2 | 7.73 × 10 −2 | 1.86 × 10 −2 | 7.63 × 10 −2 | 4.77 × 10 −2 |
Proline | 9.14 × 10 −1 | 1.76 × 10 −1 | 5.06 × 10 −2 | 9.05 × 10 −3 | 1.38 × 10 −1 | 1.23 × 10 −1 |
Pyridoxamine-5P | 4.58 × 10 −2 | 5.05 × 10 −3 | 3.32 × 10 −2 | 2.92 × 10 −3 | 3.17 × 10 −2 | 3.82 × 10 −3 |
Pyroglutamate | 2.81 × 100 | 7.22 × 10 −1 | 5.95 × 10 −1 | 8.06 × 10 −2 | 1.51 × 100 | 1.41 × 100 |
Pyruvate | 1.05 × 101 | 1.38 × 100 | 1.81 × 10 1 | 4.20 × 10 0 | 5.76 × 100 | 1.12 × 100 |
R1P | 3.62 × 10 −3 | 7.87 × 10 −4 | 2.90 × 10 −3 | 1.36 × 10 −3 | 9.68 × 10 −4 | 2.08 × 10 −4 |
R5P | 1.95 × 10 −1 | 6.88 × 10 −2 | 1.52 × 10 −1 | 6.33 × 10 −2 | 5.82 × 10 −2 | 8.74 × 10 −3 |
Ru5P | 4.04 × 10 −1 | 6.01 × 10 −2 | 2.19 × 10 −1 | 4.25 × 10 −2 | 4.48 × 10 −2 | 5.98 × 10 −3 |
RuBP | 2.92 × 10 −1 | 5.35 × 10 −2 | 2.37 × 10 −1 | 5.29 × 10 −2 | 8.39 × 10 −2 | 2.74 × 10 −2 |
S7P | 3.57 × 100 | 1.10 × 100 | 5.60 × 10 0 | 2.50 × 10 0 | 1.28 × 100 | 4.41 × 10 −1 |
Serine | 7.12 × 10 −1 | 2.63 × 10 −1 | 3.11 × 10 −1 | 9.71 × 10 −2 | 9.12 × 10 −1 | 1.01 × 100 |
Shikimate | 1.32 × 10 −3 | 5.41 × 10 −4 | 1.66 × 10 −3 | 1.13 × 10 −3 | 1.19 × 10 −3 | 7.67 × 10 −4 |
Succinate | 3.23 × 10 −1 | 7.42 × 10 −2 | 2.15 × 10 −1 | 6.81 × 10 −2 | 1.97 × 10 −1 | 4.21 × 10 −2 |
Threonine | 8.39 × 10 −1 | 1.99 × 10 −1 | 2.95 × 10 −1 | 8.09 × 10 −2 | 3.52 × 10 –1 | 2.68 × 10 −1 |
TMP | 2.71 × 10 −2 | 6.61 × 10 −3 | 2.23 × 10 −2 | 6.80 × 10 −3 | 3.57 × 10 −2 | 2.71 × 10 −3 |
Tryptophan | 2.19 × 10 −1 | 4.26 × 10 −2 | 6.95 × 10 −2 | 7.88 × 10 −3 | 8.85 × 10 −2 | 1.18 × 10 −2 |
Tyrosine | 3.04 × 100 | 6.83 × 10 −1 | 6.09 × 10 −1 | 6.31 × 10 −2 | 5.62 × 10 −1 | 2.60 × 10 −1 |
UDP-Glc | 2.24 × 100 | 2.22 × 10 −1 | 6.98 × 10 −1 | 7.48 × 10 −2 | 1.05 × 100 | 1.97 × 10 −1 |
UMP | 7.44 × 10 −1 | 1.52 × 10 −1 | 6.05 × 10 −1 | 2.43 × 10 −1 | 7.79 × 10 −1 | 1.58 × 10 −1 |
Uridine | 2.16 × 10 −2 | 9.04 × 10 −3 | 5.21 × 10 −2 | 3.01 × 10 −2 | 8.93 × 10 −2 | 2.92 × 10 −2 |
UTP | 5.54 × 10 −1 | 1.49 × 10 −1 | 3.19 × 10 −1 | 4.17 × 10 −2 | 1.75 × 10 −1 | 4.96 × 10 −2 |
Valine | 9.81 × 10 −1 | 2.00 × 10 −1 | 2.09 × 10 −1 | 3.94 × 10 −2 | 3.60 × 10 −1 | 2.13 × 10 −1 |
XMP | 4.73 × 10 −2 | 1.37 × 10 −2 | 8.66 × 10 −2 | 3.06 × 10 −2 | 1.01 × 10 −1 | 2.73 × 10 −2 |