Skip to main content
. 2014 Jun 4;307(2):C140–C149. doi: 10.1152/ajpcell.00032.2014

Fig. 3.

Fig. 3.

Human, but not mouse, satellite cells express MMP-14; MMP-14 expression in human satellite cells is dependent on contact with collagen I. A: RT-PCR for candidate MMP mRNAs. GAPDH was used as a positive control. B and C: cross sections of collagen I matrices on which human (B) or mouse (C) satellite cells were seeded and allowed to invade for 90 h. Sections were stained for MMP-14 (green) and then labeled with phalloidin 594 (red) and DAPI (blue); note the differential MMP-14 expression in human cells based on location adjacent to the collagen matrix. D: cross section of uninjured mouse tibialis anterior (TA) stained for Pax7 (red), MMP-14 (green), and DAPI (blue). E: cross section of mouse TA 5 days after BaCl injury showing high local expression between nascent myofibers in the area of injury. MMP-14 (green), DAPI (blue). F: coculture of ROSAmTmG mouse satellite cells (red) and unlabeled mouse fibroblasts on a collagen I matrix; all cells were stained for MMP-14 expression (green.) G: human satellite cells were seeded onto gelatin-coated coverslips and analyzed for MMP-14 (green) and integrin-β1 (red) colocalization.