Fig. 5.

MMP-14 is necessary for invasion by human satellite cells. A-D: transfection with MMP-14 siRNA (A and B; B is inset from A), but not scrambled control siRNA (C and D; D is inset from C), reduced invasion by human satellite cells. E: this decrease in invasion was dose dependent. F: knockdown of MMP-14 protein was confirmed by Western blotting; IP90 was used as a loading control. Protein lysates from three separate experiments are shown. G: the ratios of invading cells for control siRNA and MMP-14 siRNA were quantified as described above in 3D collagen culture. **P < 0.005.