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. 2014 May 14;307(2):C169–C179. doi: 10.1152/ajpcell.00305.2013

Fig. 1.

Fig. 1.

Fibrinogen (Fg)-induced permeability of mouse brain endothelial cells (MBECs). A and B: permeability of MBECs to Lucifer yellow (LY) and BSA tagged with Alexa Fluor 647 (BSA-647) in the presence of PBS in medium (control), 4 mg/ml fibrinogen (Fg4), 4 mg/ml Fg + 100 μM methyl-β-cyclodextrin (Fg4 + MβCD), or 100 μM MβCD. C and D: permeability of MBECs to LY and BSA-647 in the presence of PBS in medium (control), 4 mg/ml Fg (Fg4), 4 mg/ml Fg + 500 nM myriocin (Fg4 + myriocin), or 500 nM myriocin. Fluorescence intensity of each dye in samples collected from lower chambers of the Transwell system after 20, 40, 60, and 120 min was measured by a microplate reader (488-nm excitation and 520-nm emission for LY; 650-nm excitation and 668-nm emission for BSA-647). Results are expressed as ratio of fluorescence intensity of each dye in the lower chamber to fluorescence intensity of the respective dye in the original sample at the end of the experiment. Values are means ± SE; n = 4. *P < 0.05 vs. control. †P < 0.05 vs. Fg4 + MβCD or Fg4 + myriocin.