Table 1.

Amounts of tracers in lower chambers of the Transwell system at 10, 20, 40, 60, and 120 min

	Amount of Tracer, μg
	10 min	20 min	40 min	60 min	120 min
	Lucifer yellow (initial amount 0.069 μg)
Control	0.0091	0.0194	0.0295	0.0382	0.0421
Fg4	0.0138	0.0302	0.0396	0.0429	0.0457
Fg4 + MβCD	0.0113	0.0216	0.0327	0.0407	0.0447
MβCD	0.0100	0.0212	0.0301	0.0369	0.0417
	BSA-Alexa Fluor 647 (initial amount 0.034 μg)
Control	0.0009	0.0018	0.0060	0.0104	0.0140
Fg4	0.0026	0.0057	0.0117	0.0168	0.0192
Fg4 + MβCD	0.0016	0.0036	0.0077	0.0124	0.0145
MβCD	0.0009	0.0025	0.0067	0.0112	0.0142
	Lucifer yellow (initial amount 0.069 μg)
Control	0.0067	0.0097	0.0247	0.0390	0.0452
Fg4	0.0056	0.0218	0.0358	0.0449	0.0508
Fg4 + myriocin	0.0062	0.0181	0.0332	0.0421	0.0495
Myriocin	0.0055	0.0074	0.0238	0.0383	0.0460
	BSA-Alexa Fluor 647 (initial amount 0.034 μg)
Control	0.0033	0.0059	0.0135	0.0150	0.0167
Fg4	0.0045	0.0096	0.0205	0.0239	0.0268
Fg4 + myriocin	0.0028	0.0071	0.0158	0.0185	0.0204
Myriocin	0.0013	0.0052	0.0124	0.0163	0.0180

Amount of Lucifer yellow and BSA-Alexa Fluor 647 that crossed the mouse brain endothelial cell layer treated with PBS (control), 4 mg/ml fibrinogen (Fg4), 4 mg/ml fibrinogen in the presence of methyl-β−cyclodextrin (Fg4 + MβCD), or myriocin (Fg4 + myriocin), or MβCD or myriocin alone.