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Effects of 1 and 10 μM H-89, a selective inhibitor of basophilic kinases, on AQP2 phosphorylation at Ser256 in rat IMCD suspensions. After 25 min of preincubation with inhibitor or vehicle, desmopressin (dDAVP, 1 nM or vehicle) was added, and the samples were incubated for 15 min in the continued presence of the kinase inhibitor or vehicle. Vehicle was 0.5% (final concentration) DMSO. *P < 0.01.
