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Effects of 5 μM GSK-650394, a selective inhibitor of serum/glucocorticoid-regulated kinase (SGK), on AQP2 phosphorylation at Ser256 in rat IMCD suspensions. After 25 min of incubation with inhibitor or vehicle, dDAVP (1 nM or vehicle) was added, and the samples were incubated for 15 min in the continued presence of the kinase inhibitor or vehicle. Vehicle was 0.5% (final concentration) DMSO. #P < 0.1.
