Fig. 8.

Vasopressin-regulated sites with demonstrated phosphorylation by PKA, SGK, and/or CAMK2. Phosphorylation sites identified in this study were cross-referenced with 2 phosphoproteomic studies that revealed phosphorylation sites physiologically regulated by AVP: asterisk indicates sites from Bansal et al. (1), while all other sites were quantified by Hoffert et al. (23). Physiologically regulated sites are juxtaposed with a heat map of the corresponding phosphorylation responses from the 3 kinases. Sites shown in red were, on average, significantly upregulated by AVP in their respective study; sites shown in green were, on average, significantly downregulated. A: peptides with defined log₂ ratios. B: peptides with phosphorylation that was not detectable without the kinase incubation and, thus, with a log₂ (kinase/vehicle) of infinity. Peptide sequences have been extended (identified sequences are to the right of the period) to reveal the 6 amino acids upstream from the phosphorylation site.