Table 7.
Upregulated phosphoproteins: subcellular fractions
| Differential Centrifugation Fraction* |
|||||||
|---|---|---|---|---|---|---|---|
| Accession No. | Gene Symbol | Protein Name | 1K | 4K | 17K | 200K | 200K Supernatant |
| NP_001008300 | Rnmt | mRNA cap guanine methyltransferase | Present | Present | |||
| NP_001009693 | Thrap3 | Thyroid hormone receptor-associated 3 | Present | Present | |||
| NP_001011991 | Ndrg1 | NDRG1 | Present | Present | Present | ||
| NP_001029312 | Baiap2l1 | Brain-specific angiogenesis inhibitor | Present | ||||
| NP_001100446 | Ppl | Periplakin | Present | Present | Present | Present | Present |
| NP_001129138 | Lrrc47 | Leucine-rich repeat-containing 47 | Present | Present | Present | ||
| NP_037041 | Aqp2 | AQP2 | Present | Present | Present | Present | |
| NP_062220 | Slc14a2 | Urea transporter 2 isoform 1 | Present | Present | Present | ||
| NP_446368 | Trim28 | Transcription intermediary factor 1β | Present | ||||
| NP_808877 | Slc14a2 | Urea transporter 2 isoform 2 | Present | Present | Present | Present | |
| XP_001069984 | Tnks1bp1 | Tankyrase 1-binding protein 1 | Present | Present | Present | Present | |
Bold font indicates proteins predominantly phosphorylated by PKA in vitro; italicized font indicates proteins most strongly phosphorylated by CAMK2 in vitro (based on Fig. 8). Ser256 of AQP2 was phosphorylated nearly equally by PKA and CAMK2.
Fractions were obtained by differential centrifugation as described in methods and are designated as follows: 1K, 1,000-g pellet; 4K, 4,000-g pellet; 17K, 17,000-g pellet; 200K, 200,000-g pellet; 200K Supernatant, supernatant from 200,000-g spin. Gene Ontology (GO) analysis of all proteins (phosphorylated and nonphosphorylated) revealed the fractions in which individual GO cellular component terms are maximal as follows (percentage of total in fraction): 1K, nucleus (35.1), intermediate filament (3.0), tight junction (2.0), myosin complex (2.0); 4K, mitochondrion (18.6), early endosome (1.8), cell cortex (1.5); 17K, plasma membrane (20.8), endoplasmic reticulum (11.0), Golgi apparatus (7.2), endosome (4.0); 200K, ribonucleoprotein complex (11.6), ribosome (7.6), proteasome complex (5.8), microtubule (4.4); 200K supernatant, cytosol (13.9), cytoskeleton (13.3), extracellular space (2.5), actin cytoskeleton (3.1).