Table 1.
Primer sequences for CFTR and VIP receptor expression detection by RT-PCR in tracheal epithelial cells
| VIP Receptor | Primer Sequence (5′→3′) | Product Size, bp | Tm, °C | |
|---|---|---|---|---|
| VPAC1 | Forward: CTCATCGGCTGGGGAGTGCC | 910 | 60 | |
| Reverse: CCGACGGGGCGGAACCTTTT | ||||
| VPAC2 | Forward: AGGCGGAGAGGGCGATAGGC | 866 | 60 | |
| Reverse: ACTGGGGATGCCCCATCCGA | ||||
| PAC1 | Forward: GGGCCAACGACCTGATGGGC | 944 | 60.8 | |
| Reverse: GGAGCGGGCCAGCCGTAAGT | ||||
| Positive control (PKCα) | Forward: TGCCGGCCAGTGGATGGTAT | 651 | 57.9 | |
| Reverse: TGCACATCCCAAAGTCGGCG | ||||
| CFTR | Forward: CGCGCAGCAACATGATCGCC | 902 | 60 | |
| Reverse: TGGACTCCTGCCTTCAGATTCCAGT |
Expected product sizes in base pairs (bp) and annealing temperature (Tm) used in RT- PCR are indicated.