Fig. 2.

AT₁ receptor-mediated uptake of FITC-ANG II in wild-type mPCT cells. Monolayers of wild-type mPCT cells grown on glass coverslips were incubated with FITC-ANG II (10 nM) at 37°C for 30 min, washed, and imaged (see materials and methods for details). A, D, and G: FITC-ANG II (A; green)-, DAPI-stained (D; red), or merged image (G) of A and D. Uptake of FITC-ANG II was seen in the cytoplasm and pronuclear regions of the cells. B, E, and H: effect of losartan, which largely abolished the uptake of FITC-ANG II. C, F, and I: effect of PD123319, which did not significantly block the uptake of FITC-ANG II. J: semiquantitated data on the uptake of FITC-ANG II in control (untreated), losartan- or PD123319-pretreated mPCT cells, expressed as arbitrary fluorescence units (AFU). Magnification: ×100. **P < 0.01 vs. untreated mPCT cells.