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. 2014 May 28;307(2):F159–F171. doi: 10.1152/ajprenal.00546.2013

Fig. 7.

Fig. 7.

Polymerase (DNA-directed) δ-interacting protein 2 (Poldip2) regulates myofibroblast differentiation via Nox4. A–F: kidney fibroblasts were infected with AdPoldip2 or adenovirus with green fluorescent protein (AdGFP) over a 48-h period, and the relative effects of overexpression of Poldip2 on Nox4 protein (A–C), NADPH oxidase activity (D), and α-SMA and Fn-EIIIA (A, E, and F) expression were examined. Increased expression of Poldip2, as determined by Myc and Poldip protein by immunoblot analysis, resulted in enhanced expression of Poldip2 and Nox4 protein and NADPH oxidase activity as well as the myofibroblast differentiation markers α-SMA and Fn-EIIIA relative to control. G–L: infection of kidney fibroblasts with AdNox4 significantly increased Nox4 and Poldip2 protein (G–I), NADPH oxidase activity (J), and the myofibroblast differentiation markers α-SMA and Fn-EIIIA (G, K, and L) relative to control (AdGFP). *P < 0.05 vs. AdGFP vs. control.