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Linescan technique applied to mitochondria in rat cardiac myocytes. A: definition of the mPTP ROS threshold as an average time over population of mitochondria (shown by a dashed red line) in myocyte stained with fluorescent mitochondrial probe for the membrane potential to induce the mPTP (shown by arrow) (491, 493). B: dependence of the mPTP induction in the cell on ambient molecular oxygen.
