FIGURE 5.

Relationship between mitochondrial ΔΨ and H₂O₂ production supported by succinate or NADH-linked respiratory substrates. A: rat heart mitochondria oxidizing succinate. The Δψ level was varied by adding different concentrations of uncoupler SF6847 (black squares and solid line), malonate (white squares), or 100 μM ADP and 5 mM P (triangle). Dashed line, the state 3 Δψ level. [From Korshunov et al. (240), with permission from Elsevier.] B: rat brain mitochondria oxidizing glutamate + malate or α-ketoglutarate. Differences in ΔΨ were generated by adding various concentrations of uncoupler FCCP ranging from 0 to 80 nM. Alternatively, a decrease in Δψ was induced by adding 0.8 mM ADP to mitochondria. [From Starkov and Fiskum (419), with permission from John Wiley & Sons.]