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Δψ loss in a significant fraction of mitochondria, caused by hypoxia/reoxygenation. Depolarized mitochondria (red-fluorescence ”holes“; bottom panels) are associated with increased ROS (green; bottom left panel). Hypoxic PC or pharmacological preconditioning (PC), represented by diazoxide (Dz), prevents mitochondrial depolarization, and 5-hydroxydecanoate (5HD) accentuates the loss. [From Hoffman et al. (189).]
