Table 1.

Effect of fetal number and PCUN on the expression of IGF1, IGF1R, ACTHR, StAR, 3βHSD, and CYP11B mRNA relative to RpPO in the fetal adrenal

	IGF1:RpPO	IGF1R:RpPO	ACTHR:RpPO	StAR:RpPO	3βHSD:RpPO	CYP11B:RpPO
Singleton
Control	0.022 ± 0.002	0.15 ± 0.02	0.50 ± 0.03	5.3 ± 0.4	27.0 ± 1.9	5.9 ± 0.6
PCUN	0.019 ± 0.003	0.12 ± 0.01	0.50 ± 0.04	4.7 ± 0.3	28.6 ± 3.1	6.2 ± 0.8
PIUN	0.023 ± 0.007	0.10 ± 0.02	0.40 ± 0.03	5.5 ± 0.8	25.9 ± 4.4	6.1 ± 0.1
Twin
Control	0.022 ± 0.002	0.15 ± 0.03	0.36 ± 0.03*	4.8 ± 0.5	22.5 ± 2.7	5.1 ± 0.7
PCUN	0.028 ± 0.003	0.16 ± 0.03	0.43 ± 0.03*	4.4 ± 0.8	24.5 ± 3.2	5.9 ± 0.7
PIUN	0.028 ± 0.002	0.18 ± 0.02	0.38 ± 0.03*	5.3 ± 0.5	22.5 ± 1.7	6.1 ± 0.3

Values are means ± SE. PCUN, periconceptional nutrition; PIUN, preimplantation undernutrition.

^*Significant difference overall between singleton and twin fetuses (*P < 0.05).