Fig. 1.

Culture geometry and boundary conditions used in experiments. A: Lab-Tek II culture chamber containing Teflon molds and long-axis constrained (LAC) stainless steel mesh. B: assembled culture chambers for all boundary conditions (left), stainless steel meshes (middle), and 3-dimensional (3D) collagen cultures after removal of Teflon molds (right). The region that was imaged and quantified by laser scanning confocal microscopy is shown as a white box. The Lab-Tek chambers used for the hexahedral constructs measured 49 × 19 × 11 mm, whereas the chambers used in the circular constructs measured 23 × 19 × 11 mm.