Fig. 2.

Obesity with or without lymphatic injury decreases lymph node ^99mTc uptake. A: representative heat maps of control and lymphedematous mouse tails injected with ^99mTc for lymphoscintigraphy. White arrows point to sacral lymph nodes. B and C: quantification of decay-adjusted ^99mTc uptake by sacral lymph nodes in control (B) and lymphedematous (C) mice. D: quantification of peak nodal uptake in control and lymphedematous animals 6 wk postoperatively. *P < 0.05. E: representative photomicrographs (×20 magnification) and quantification of lymphatic vessel endothelial hyaluronan receptor (LYVE)-1⁺ vessels (left graph) and LYVE-1⁺ vessel area (right graph) in control and lymphedematous tail sections 6 wk postoperatively.