Figure 3.

PTRF overexpression compromises 3T3-L1 differentiation. 3T3-L1 preadipocytes were transduced with either empty lentivirus (control) or lentivirus encoding PTRF (PTRF) and subsequently differentiated into adipocytes. A) Immunoblot for PTRF expression. B) PTRF mRNA levels in adipocytes. C) Oil Red-O stained adipocytes to visualize lipid accumulation at ×1 (top panels) and ×40 view (bottom panels). D) Flow cytometric analysis of adipocytes stained with Nile Red. Percentages of subpopulations of nondifferentiated preadipocytes (red) and lipid-laden adipocytes (green) are superimposed. Data are representative of 3 replicates. E) Time-lapse analysis of CEBPβ and CEBPδ mRNA expression during early adipogenesis. Preadipocytes were stimulated to differentiate at time 0 and harvested at the indicated time points. Individual assessments are means ± sem of 5 replicates. *P < 0.05.