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Serum iron levels in Fpn-KO (open bars) and WT (solid bars) mice following 30 min exposure of duodenal loops to iron. Duodenal loops in male mice (8–12 wk old) were infused with either saline (control), 500 μM iron as FeNTA2, or 500 μM iron as nanoFe3+. Data represent means ± sd. Numbers in each group are as follows: saline control, n = 6 WT and n = 7 Fpn KO; FeNTA2, n = 7 WT and n = 7 Fpn KO; nanoFe3+, n = 10 WT and n = 9 Fpn KO. *P ≤ 0.04, **P = 0.007.
