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. 2014 Aug;28(8):3633–3644. doi: 10.1096/fj.13-248708

Figure 3.

Figure 3.

Activation of A2BR by sialorphin is dependent on Hif-1a activation. A) Representative immunoblot to determine protein expression of Hif-1a, A2BR, and GAPDH in rat CSM cells with 400 nM sialorphin alone, or in combination with Hif-1a inhibitor for 8 h. B, C) Densitometric analysis of 3 separate experiments to determine expression of A2BR and GAPDH from 6 separate experiments to determine expression of Hif-1a. Expression of Hif-1a and A2BR were normalized to the housekeeping gene GAPDH. Data represent mean ± sd fold change in expression of Hif-1a (B) and A2BR (C) caused by increasing Hif-1a inhibitor concentration relative to untreated controls (CSM cells without sialorphin or Hif-1a inhibitor treatment). *P < 0.05 vs. controls.