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. 2014 Jul 4;7:313. doi: 10.1186/1756-3305-7-313

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Copyright © 2014 Hao et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Recognition of recombinant and native Ts-Pmy (~102 kDa) by mAb 9G3, as demonstrated by Western blot. M, protein marker; Lane 1, adult extracts (5 μg); Lane 2, ML extracts (5 μg); Lane 3, NBL extracts (5 μg); Lane 4, rTs-Pmy with a His-tag (0.5 μg); Lane 5,BSA(1 μg); Lane 6, irrelevant recombinant Ts-Hsp70 control (1 μg). After transfer of the proteins to the nitrocellulose membrane, the membrane was incubated with mAb 9G3 (0.2 μg/ml). IRDye 800CW-labeled goat anti-mouse IgG was used as the secondary antibody.