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. 2014 Jul 9;14:495. doi: 10.1186/1471-2407-14-495

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Copyright © 2014 Lehner et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Significant downregulation of Dlk1, Meg3 and Meg8 in GCTSCs. (A) Expression of Dlk1, Meg3, Meg8, Rtl1 and Dio3 was analyzed by RT-qPCR in GCTSCs (n = 5) and MSCs (n = 5). Data were normalized on the basis of the ribosomal protein L19 (RPL19) expression in the corresponding sample. Data are presented as mean ± SD. (*p < 0.05 **p < 0.01 determined by Mann–Whitney-U test). (B) IG-DMR copy number assay. The IG-DMR copy number was determined by RT-qPCR in MSCs, GCTSCs and osteoblasts and calculated using the genomic RNAse P region as reference.