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. 2014 May 30;47(1):22. doi: 10.1186/0717-6287-47-22

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© ACHARYA et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Bar diagram used for finding the detection limit of the assay. Log concentration of the dilution series plotted with their corresponding Threshold Cycles. Series 1: Threshold Cycles; Series 2: Log concentration of viral load; CTN10: 10 HIV-1 RNA copies/ml; CTN30: 30 HIV-1 RNA copies/ml; CTN50: 50 HIV-1 RNA copies/ml; CTN100: 100 HIV-1 RNA copies/ml; CTN500: 500 HIV-1 RNA copies/ml.