Table 1.
Precision of the SYBR Green Real time PCR assay determined in this study
| Reference concentration (copies/ml) | Measured concentration (copies/ml) | Standard deviation (SD) | Coefficient of variation (CV) |
|---|---|---|---|
| 52 | 50 | 1.65 | 0.0325 |
| 124 | 120 | 3.06 | 0.0251 |
| 344 | 345 | 0.47 | 0.0014 |
| 871 | 863 | 5.42 | 0.0063 |
| 1,235 | 1,250 | 10.14 | 0.0082 |
| 16,383 | 16,358 | 17.68 | 0.0011 |
| 267,816 | 267,098 | 507.70 | 0.0019 |
| 31,293 | 31,299 | 4.48 | 0.0001 |
| 541 | 534 | 4.71 | 0.0088 |
| 1,476 | 1,466 | 6.60 | 0.0045 |
| 4,572 | 4,555 | 12.26 | 0.0027 |
| 2571,210 | 2569,050 | 1,526.88 | 0.0006 |
| 76 | 89 | 9.43 | 0.1145 |
| 43,110 | 43,135 | 18.15 | 0.0004 |
| 5,447 | 5,414 | 23.10 | 0.0043 |
| 21,312 | 21,393 | 57.28 | 0.0027 |
| 96 | 94 | 1.65 | 0.0174 |
| 635,469 | 635,592 | 86.97 | 0.0001 |
| 626 | 620 | 4.48 | 0.0072 |
| 54,886 | 54,853 | 23.81 | 0.0004 |
| 8,742 | 8,781 | 27.58 | 0.0031 |
| 917,838 | 917,648 | 133.88 | 0.0001 |
| 74,511 | 74,552 | 29.23 | 0.0004 |
| 944 | 965 | 15.32 | 0.0161 |
| 9,648 | 9,984 | 237.82 | 0.0242 |
The standard deviation and the coefficient of variation were determined using data from 10 independent experiments.