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. 2014 May 16;47(1):17. doi: 10.1186/0717-6287-47-17

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© Yan et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of egg extract on the neuromuscular transmission in mouse isolated phrenic nerve-hemidiaphragm preparations. Legend: The neurotoxicity of the egg extract was demonstrated by detecting its effect on the amplitude of phrenic muscle contraction caused by the electrical stimulation of phrenic nerve. (A) control; (B) test, adding the egg extract at a concentration of 0.25 mg/mL after stable contraction in Tyrode’s solution for about 20 min; (C) washing the test preparation with Tyrode’s solution after blockade.