Table 1.
The expression of cell-specific markers in freshly isolated and pre-induced hPBMCs.
| Markers | Groups | T | P-value | |
|---|---|---|---|---|
| Before Pre-induction(mean±S.D.) | After Pre-induction(mean±S.D.) | |||
| Vimentin | 47.53±5.84 | 78.97±12.43 | -3.38 | 0.08 |
| Nestin | 1.73±1.86 | 5.23±3.03 | -3.50 | 0.07 |
| GFAP | 0.08±0.14 | 2.40±1.31 | -3.34 | 0.08 |
| MAP-2 | 0.20±0.26 | 5.70±3.04 | -3.29 | 0.08 |
| β-tubulin III | 0.63±0.50 | 7.53±7.32 | -1.75 | 0.22 |
| Synapsin | 0.43±0.31 | 4.13±4.65 | -1.39 | 0.30 |
| Rhodopsin | 1.50±1.78 | 26.43±6.83 | -5.68 | 0.03* |
| CD45 | 56.73±19.27 | 73.70±20.91 | -2.21 | 0.16 |
| CD3 | 37.70±11.56 | 29.33±14.39 | 4.01 | 0.06 |
| CD16 | 13.30±13.19 | 5.73±5.89 | 0.74 | 0.54 |
| CD19 | 13.53±8.05 | 1.23±0.32 | 2.55 | 0.13 |
Flow cytometry results are expressed as mean ± S.D. and group differences by T and P-values. *P< 0.05, significantly different between groups. Before Pre-induction: freshly iso-lated human peripheral blood mononuclear cells (hPBMCs). After Pre-induction: cells cultured for 4 days in a retinal tissue co-culture system with neural stem cell medium.