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. 2014 Mar 15;3(3):472–484. doi: 10.1002/cam4.222

Figure 1.

Figure 1

Effect of 15-LOX-1 on colon cancer cell survival under hypoxia. (A–C) The indicated cell lines were transfected with mock, Ad-15-LOX-1, or control Ad-luciferase and cultured under hypoxia for 24 h. Cells were harvested and 15-LOX-1 mRNA levels were measured by real-time RT-PCR for 15-LOX-1. Values are mean ± SD. *P < 0.0001, compared to Mock or Ad-luciferase group, ANOVA. (D) Cells were transfected and cultured as described as panels A–C but for 48 h, and then harvested for 15-LOX-1 protein expression level measurements by Western Blotting. (E-G) The indicated cell lines were transfected with mock, Ad-15-LOX-1, or Ad-luciferase and cultured under hypoxia for 5 days before cell survival (ratio of MTT measured values to mock transfected cells cultured simultaneously under ideal conditions) was measured by MTT assay. Data are presented as ratios to the cells transfected with mock. Values are mean ± SD. *P < 0.0001 compared with the Ad-luciferase group.