Fig. 8.

Autophagy inhibitor 3-methyladenine (3-MA) attenuated mechanical ventilation-induced lung injury in mice. Mice were pretreated with autophagy inhibitor 3-MA 1 h before exposure to high tidal volume mechanical ventilation (MV) for 2 h. Low tidal volume ventilation was used in the control group. A: effects of 3-MA on LC3-II protein expression in the lung following mechanical ventilation. Left, representative Western blots for LC3-I and LC3-II; right, protein quantification by densitometry. Bar graph shows the relative abundance of LC3-II protein (normalized to that of GAPDH) from 3 experiments. B: protein concentrations in BAL fluid. C: ELW. D: polymorphonuclear neutrophil (PMN) counts in BAL fluid. E: lung MPO activity. *P < 0.05 vs. control (CON) group. †P <0.05 vs. MV group; n = 6 per groups.