Table 1.
Summary of mucin matrix diffusivities when exposed to varying concentrations of nicotine
| Preloading Buffer for 15 Min | Bathing Buffer for Swelling Kinetics Measurement | Diffusivity, Cm2/S | |
|---|---|---|---|
| Nonpreloaded cells | Not preloaded | HBSS buffer | |
| [Nicotine] = 0 μM | 1. [Nicotine] = 0 nM | 1. 4.18 ± 0.76 (n = 10) | |
| 2. [Nicotine] =5 nM | 2. 3.88 ± 0.55 (n = 36) | ||
| 3. [Nicotine] =10 nM | 3. 2.24 ± 0.14* (n = 14) | ||
| 4. [Nicotine] =50 nM | 4. 1.81 ± 0.38* (n = 19) | ||
| 5. [Nicotine] =100 nM | 5. 1.37 ± 0.16* (n = 14) | ||
| 6. [Nicotine] =1 μM | 6. 1.48 ± 0.14* (n = 7) | ||
| Nicotine preloaded cells | HBSS buffer | HBSS buffer | |
| 1. [Nicotine] = 0 nM | [Nicotine] = 0 μM | 1. 4.18 ± 0.76 (n = 10) | |
| 2. [Nicotine] = 10 nM | 2. 2.22 ± 0.13* (n = 21) | ||
| 3. [Nicotine] = 100 nM | 3. 1.20 ± 0.025* (n = 12) | ||
| 4. [Nicotine] = 1 μM | 4. 1.22 ± 0.042* (n = 9) | ||
| Nicotine preloaded isolated granules | Intracellular buffer | Intracellular buffer | |
| 1. [Nicotine] = 0 nM | [Nicotine] = 0 μM | 1. 4.66 ± 0.39 (n = 11) | |
| 2. [Nicotine] = 100 nM | 2. 1.27 ± 0.69* (n = 21) | ||
| 3. [Nicotine] = 1 μM | 3. 1.56 ± 0.50* (n = 13) |
Diffusivities of means±s.d. are provided for each nicotine concentration.
*
Significantly different from control (nicotine-free) (P < 0.0001; Student's t-test); n represents the number of cells or granules investigated in the experiments.