Skip to main content
. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Immunity. 2014 Jun 12;40(6):936–948. doi: 10.1016/j.immuni.2014.05.007

Fig. 2. OASL expression provides cellular antiviral activity.

Fig. 2

(A) OASL expression inhibits VSV infection. Cells were infected with VSV-GFP at 1 m.o.i. GFP florescence was observed under florescence microscope 8 h post-infection. Representative micrograph from at least three separate experiments is shown. (B–C) Expression of OASL reduces VSV replication. Cells were infected with VSV-GFP at the indicated m.o.i for 8 h followed by IB using GFP antibody (B). Supernatants from similarly infected cells (5 m.o.i) were used for plaque assay on BHK21 cells (C). (D) OASL expression inhibits SeV infection. SeV infected (24 h) cells were analyzed by IB using antibody against SeV C protein. Densitometric analysis of the protein bands are shown below. (E–G) OASL expression inhibits RSV, DENV and HSV-1 replication. Cells were infected with RSV (3 m.o.i) for 48 h, followed by plaque assay on Hep2 cells (E). Cells were infected with type 2 dengue virus (DENV) at indicated doses for 48 h followed by qRT-PCR for DENV specific RNA (F). Cells were infected with HSV-1 at 5 m.o.i. for 24 h followed by plaque assay on Vero cells (G).