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. 2014 May 3;14:37. doi: 10.1186/1472-6750-14-37

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Copyright © 2014 Buntru et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Optimization of reaction conditions in batch mode. Effects on eYFP yield caused by (a) magnesium acetate concentration, (b) potassium acetate concentration, (c) mRNA concentration, (d) presence (+) or absence (-) of poly(A) sequence. Translation reactions were carried out using capped GAA_Omega_eYFP-His mRNA as the template at 25°C and 500 rpm for 18 h. The relative fluorescence intensities are shown. Mean values were calculated from three independent translation experiments.