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. 2014 May 3;14:37. doi: 10.1186/1472-6750-14-37

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Copyright © 2014 Buntru et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Translation of firefly and Renilla reniformis mRNAs in the BYL and WGE systems. Reactions were carried out with 2 μg capped mRNA as the template at 25°C and 500 rpm for 18 h. (a) Bar graphs show luciferase activity expressed as relative luciferase units (RLU). Data represent the averages and standard deviations of six independent translation experiments. (b) Typical result of FFLuc translation in the BYL and WGE systems, in which 1 μl of reaction mixture was combined with 50 μl luciferase assay buffer: FFLuc standard with 0.1 μg/μl (1), pIVEX1.3_FFLuc-His in BYL (2), pIVEX1.3_FFLuc-His in WGE (3), no template control in BYL (4), no template control in WGE (5).