Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 May 3;14:37. doi: 10.1186/1472-6750-14-37

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Buntru et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Translation of eYFP mRNAs in BYL and WGE systems in dialysis mode. Reactions were carried out with 3 μg capped mRNA as the template at 25°C and 900 rpm for 18 h and 24 h, respectively. The amount of fluorescent protein (a) was determined by comparison with an eYFP standard curve generated by measuring different eYFP concentrations in BYL translation reactions without a mRNA template. Data represent the averages and standard deviations of six independent translation experiments. (b) SDS-PAGE of the BYL and WGE translation reactions. In each case 10 μl of the translation reactions were loaded on a 4-12% gradient gel. Lane 1: GAA_Omega_eYFP-His in BYL; lane 2: no template control in BYL; lane 3 GAA_Omega_eYFP-His in 5Prime WGE; lane 4: no template control in 5Prime WGE; lane 5: GAA_Omega_eYFP-His in Promega WGE; lane 6: no template control in Promega WGE; M: molecular weight marker.