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. 2014 Apr 5;14:243. doi: 10.1186/1471-2407-14-243

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Copyright © 2014 Huang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Examples of quantitative analysis by SILAC. SILAC quantitative analysis of phosphopeptides was used to identify DNP-mediated effectors. Some peptides were quantified and showed fold differences between the control and DNP-treated cells. A, A representative mass spectrum showing a peptide pair (GLRDpSHpSpSEEDEASSQTDLSQTISK from Rho GTPase) with a doubly charged ion differing by 3 Da and exhibiting a H/L (of XIC) ratio of 0.35802; B, A representative mass spectrum a peptide pair (KFNMIPFEHRpSGGK from torsin-1A protein 1) with triply charged ions differing by 2 Da and exhibiting a H/L (of XIC) ratio of 3.2498.