Skip to main content
. 2014 Apr 5;14:243. doi: 10.1186/1471-2407-14-243

Figure 7.

Figure 7

DNP induces motility and invasion through LYRIC phosphorylation. The 6-10B cells were transfected using pcDNA3.1, pcDNA3.1-LYRIC or pcDNA3.1-LYRICmutant, and then the stably transfected 6-10B-mock, 6-10B-LYRIC and 6-10B-LYRICmutant cell lines were obtained by selection for G418 resistance. LYRIC and phospho-LYRIC expressions were detected in these cells with or without DNP treatment (A). β-actin was used as a loading control. Motility (B) and invasion (C) of 6-10B-mock, 6-10B-LYRIC, and 6-10B-LYRICmutant cells were determined in these cells. D, CNE1 cells were treated with DNP at 6 μM, and LYRIC and phospho-LYRIC expressions were detected using immunoblotting (a), and motility (b) and invasion (c) were measured using Boyden chamber in these cell samples. Data are presented as means ± SD from 3 independent experiments. Results were analyzed using one-way ANOVA with post-hoc Dunnett’s test (*, p < 0.05).