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Luciferase assay for mouse fgf10 and ANF promoters. Both chicken and moa Tbx5 activate the fgf10 and ANF promoters in a similar dose dependent manner. 1x, 2x and 10x amounts of vectors containing chicken and moa tbx5 were transfected into HEK293T cells with fgf10 and ANF-luciferase promoter constructs, and luciferase activity was measured. Both chicken and moa tbx5 were equally effective in the activation of fgf10 and ANF promoter elements.
