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. Author manuscript; available in PMC: 2014 Jul 17.
Published in final edited form as: Nat Biotechnol. 2013 Dec 1;32(1):84–91. doi: 10.1038/nbt.2754

Fig. 2. Requirement of morphogens for lung field induction from hPSCs.

Fig. 2

Effect of removing individual factors or blocking signaling pathways during the ‘ventralization’ stage (d6–d15) on the expression of FOXA2 and NKX2.1 in RUES2 cells cultured according to the protocol shown on the upper left of the figure. 10x tile scans of 9 (3×3) contiguous fields. Immunofluorescence images represent reproducible results from 3 independent experiments.